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J Gen Virol 17 (1972), 49-54; DOI 10.1099/0022-1317-17-1-49
© 1972 Society for General Microbiology
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The Application of DEAE-cellulose Column Chromatography to the Selection of S+ Revertants and Determination of Reversion Frequency in Populations of S- Mutants of Fowl Plague Virus

A. B. Germanov, M. I. Sokolov and N. A. Parasiuk

Ivanovsky Institute of Virology, Moscow, USSR

A method has been developed for selection of revertants present at low frequency in a population of fowl plague virus. It was based on the differences in elution from DEAE-cellulose columns of the original large-plaque variant (S+) and polycation-dependent plaque-size mutants (S-). It was possible to estimate low frequencies of revertants in this way.

Received 24 February 1972; accepted 8 June 1972.




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